Olympus LSM FV1200S-IX83
Inverted Scanning Confocal Microscope
- Motorized platform
- Long-working distance condenser
- Differential Interference Contrast
FV1200 Confocal:
- 3 laser ports
- 3 confocal fluorescence detection channels (PMT’s 300 – 850 nm)
- Transmitted light detector
- High sensitivity confocal GaAsp Detectors (2x) hvilke detectioner?
- Z-axis control for IX-83
- Time controller
- FRET wizard
- Sim scanner
Laser lines:
- Laser diode 405 nm (DAPI, Photoactivation, Uncaging)
- Argon laser: 458, 488, 515 nm (CFP, GFP, YFP, Alexa 488, FITC)
- DPSS Laser: 559 nm (RFPs, Cy3, TRITC, Alexa 555/568)
- Red Diode Laser: 635 nm (Cy5, Alexa 633/647)
Objectives:
- UPLSAPO10x2 NA 0.40 WD 3.1 mm
- UPLSAPO20x NA 0.75 WD 0.60 mm
- UPLSAPO40x NA 0.95 WD 0.18 mm
- UPLSAPO60xO NA 1.35 WD 0.15 mm (oil)
Fluorescence filter cubes:
- U-FUW (Wideband Ultraviolet excitation) – UV excitation: e.g. DAPI, Hoechst, Indo
- U-FBN (Narrowband Blue excitation) – Blue excitation: e.g. FITC, GFP/ EGFP, GFP2, Alexa Green, Fluo (Calcium indicator), Calcium green
- U-FGWA (Wideband Green excitation) – Green excitation: e.g. Rhodamine/TRITC, Cy3, Texas Red, Alexa Red, RFP
- U-F61001: Triple band filterset for DAPI/FITC/Propidium Iodide
Leica TCS SP5
Inverted Scanning Confocal Microscope
- Two-photon laser
- Motorized platform
- Long-working distance condenser
- Differential Interference Contrast
Laser illumination lines:
- Argon 458/476/488/514nm,
- DPSS 561nm,
- HeNe 594nm, and
- HeNe 633nm.
Confocal detection channels:
- Five spectral detector channels for fluorescence (2 HyD & 3 PMT)
- Non-descanned Detector for two-photon.
- Three photomultiplier tubes (PMTs) for fluorescence detection
- One channel for transmitted light (bright field, differential interference contrast (DIC)).
Objectives:
- 10x (NA 0.4)
- 63x (NA 1.4) oil